Follicles once developed to an ovulable state may begin to degenerate within 3 hr and completely lose their ability to ovulate within 9 hr after the neutralization of circulating gonadotropin. It was concluded that PMS is needed continuously in the circulating blood to maintain follicles until at least 3 hr before ovulating hormone administration. Toxoplasma tachyzoites were purified from the supernatant fluid of. No ovulation occurred by hCG 9 hr after APS administration. calf serum, penicillin (100 U/mL), and streptomycin (100 g/mL) at 37C and 5 CO2. When hCG injection was delayed 3 hr after APS, the mean number of ova per ovulating rat had already decreased significantly to 14.4 as compared to 23.3 for the corresponding control group, although a similar percentage of ovulation was obtained (80 vs 86). When hCG was given simultaneously with APS, almost all of the rats ovulated. HCG was used as an ovulating hormone to test the follicular ability to ovulate. When follicles developed to an ovulable state (60–72 hr after PMS), APS was given iv at various times during this period to neutralize circulating PMS. Immature rats hypophysectomized on day 22 received PMS iv on day 26. A study was conducted to determine whether or not circulating gonadotropin is required for follicular ovulability after the follicles have grown to an ovulable state, using PMS and anti- PMS serum ( APS) in hypophysectomized immature female rats.

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